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Am J Reprod Immunol. 2015 Jun;73(6):501-6. doi: 10.1111/aji.12359. Epub 2015 Jan 21.

Cyclic stretch augments production of neutrophil chemokines, matrix metalloproteinases, and activin a in human endometrial stromal cells.

Author information

1
Obstetrics and Gynecology, The University of Tokyo, Tokyo, Japan.
2
Chemical Genetics Laboratory, RIKEN, Saitama, Japan.
3
Anti-Aging Medicine, The University of Tokyo, Tokyo, Japan.

Abstract

PROBLEM:

The aim of this study was to test our hypothesis: Contractile activity that occurs in the uterus during menstruation induces biochemical factors that enhance remodeling of the endometrium.

METHOD OF STUDY:

Cyclic stretch, mimicking contractile activity during menstruation, was applied to human endometrial stromal cells (ESC) using the Flexercell Tension system. The concentration and activity of CXCL8, CXCL1, MMPs, and activin A were measured using ELISAs and specific assays. Neutrophil chemotactic activity was evaluated using migration assays.

RESULTS:

Cyclic stretch significantly induced ESC secretion of CXCL8 and CXCL1 and neutrophil chemotaxis. Stretch also increased MMP-1, MMP-2, and MMP-3 activity, activin A secretion, and activity in ESC.

CONCLUSION:

These results indicate that the contractile activities of the uterus during menstruation contribute to the remodeling of the endometrium, by inducing chemokine secretion, MMP expression, activity, and neutrophil chemotaxis.

KEYWORDS:

CXCL1; CXCL8; endometrium; remodeling

PMID:
25605062
DOI:
10.1111/aji.12359
[Indexed for MEDLINE]

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