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Curr Opin Plant Biol. 2015 Apr;24:1-9. doi: 10.1016/j.pbi.2015.01.001. Epub 2015 Jan 17.

Unravelling plant molecular machineries through affinity purification coupled to mass spectrometry.

Author information

1
Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; CropDesign N.V., Technologiepark 21, B-9052 Ghent, Belgium.
2
Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.
3
Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium. Electronic address: geert.dejaeger@psb.vib-ugent.be.

Abstract

Rather than functioning independently, proteins tend to work in concert with each other and with other macromolecules to form macromolecular complexes. Affinity purification coupled to mass spectrometry (AP-MS) can lead to a better understanding of the cellular functions of these complexes. With the development of easy purification protocols and ultra-sensitive MS, AP-MS is currently widely used for screening co-complex membership in plants. Studying complexes in their developmental context through the isolation of specific organs and tissues has now become feasible. Besides, the tagged protein can be employed for probing other interactions like protein-DNA and protein-RNA interactions. With the tools at hand, protein-centred interaction studies will greatly improve our knowledge of how plant cells wire their functional components in relation to their function.

PMID:
25603557
DOI:
10.1016/j.pbi.2015.01.001
[Indexed for MEDLINE]

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