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Int J Biol Macromol. 2015 Apr;75:51-7. doi: 10.1016/j.ijbiomac.2014.11.047. Epub 2015 Jan 17.

The extraction process optimization of antioxidant polysaccharides from Marshmallow (Althaea officinalis L.) roots.

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Department of Food Science & Technology, Islamic Azad University, Science and Research Branch, Faculty Street, PO Box 14515.775, Tehran, Iran. Electronic address:


Response surface methodology (RSM) with a central composite rotatable design (CCRD) based on five levels was employed to model and optimize four experimental operating conditions of extraction temperature (10-90 °C) and time (6-30 h), particle size (6-24 mm) and water to solid (W/S, 10-50) ratio, obtaining polysaccharides from Althaea officinalis roots with high yield and antioxidant activity. For each response, a second-order polynomial model with high R(2) values (> 0.966) was developed using multiple linear regression analysis. Results showed that the most significant (P < 0.05) extraction conditions that affect the yield and antioxidant activity of extracted polysaccharides were the main effect of extraction temperature and the interaction effect of the particle size and W/S ratio. The optimum conditions to maximize yield (10.80%) and antioxidant activity (84.09%) for polysaccharides extraction from A. officinalis roots were extraction temperature 60.90 °C, extraction time 12.01 h, particle size 12.0mm and W/S ratio of 40.0. The experimental values were found to be in agreement with those predicted, indicating the models suitability for optimizing the polysaccharides extraction conditions.


Antioxidant activity; Aqueous extraction; DPPH radical scavenging activity; Marshmallow polysaccharide; Optimization

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