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Plant Cell. 2015 Jan;27(1):177-88. doi: 10.1105/tpc.114.131862. Epub 2015 Jan 16.

A transient receptor potential ion channel in Chlamydomonas shares key features with sensory transduction-associated TRP channels in mammals.

Author information

1
Physiology Department, Faculty of Medicine, Universidad Austral de Chile, Campus Isla Teja, Valdivia 5110566, Chile.
2
Universidad Andres Bello, Center for Bioinformatics and Integrative Biology, Faculty of Biological Sciences, Santiago 8370146, Chile.
3
Universidad Andres Bello, Center for Bioinformatics and Integrative Biology, Faculty of Biological Sciences, Santiago 8370146, Chile CINV, Faculty of Sciences, Universidad de Valparaíso, Valparaíso 2366103, Chile.
4
Biology Department, Faculty of Chemistry and Biology, Universidad de Santiago de Chile, Santiago 9160000, Chile.
5
Physiology Department, Faculty of Medicine, Universidad Austral de Chile, Campus Isla Teja, Valdivia 5110566, Chile sbrauchi@uach.cl.

Abstract

Sensory modalities are essential for navigating through an ever-changing environment. From insects to mammals, transient receptor potential (TRP) channels are known mediators for cellular sensing. Chlamydomonas reinhardtii is a motile single-celled freshwater green alga that is guided by photosensory, mechanosensory, and chemosensory cues. In this type of alga, sensory input is first detected by membrane receptors located in the cell body and then transduced to the beating cilia by membrane depolarization. Although TRP channels seem to be absent in plants, C. reinhardtii possesses genomic sequences encoding TRP proteins. Here, we describe the cloning and characterization of a C. reinhardtii version of a TRP channel sharing key features present in mammalian TRP channels associated with sensory transduction. In silico sequence-structure analysis unveiled the modular design of TRP channels, and electrophysiological experiments conducted on Human Embryonic Kidney-293T cells expressing the Cr-TRP1 clone showed that many of the core functional features of metazoan TRP channels are present in Cr-TRP1, suggesting that basic TRP channel gating characteristics evolved early in the history of eukaryotes.

PMID:
25595824
PMCID:
PMC4330573
DOI:
10.1105/tpc.114.131862
[Indexed for MEDLINE]
Free PMC Article

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