Format

Send to

Choose Destination
See comment in PubMed Commons below
J Mol Cell Cardiol. 2015 Apr;81:54-61. doi: 10.1016/j.yjmcc.2015.01.003. Epub 2015 Jan 14.

Obligatory role of neuronal nitric oxide synthase in the heart's antioxidant adaptation with exercise.

Author information

1
Department of Physiology & Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA.
2
Institut des Maladies Métaboliques et Cardiovasculaires, Institut National de la Santé et de la Recherche Médicale, Toulouse, France.
3
Department of Physiology, Cardiovascular Research Center, Temple University, Philadelphia, PA, USA.
4
Department of Physiology & Cell Biology, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA. Electronic address: ziolo.1@osu.edu.

Abstract

Excessive oxidative stress in the heart results in contractile dysfunction. While antioxidant therapies have been a disappointment clinically, exercise has shown beneficial results, in part by reducing oxidative stress. We have previously shown that neuronal nitric oxide synthase (nNOS) is essential for cardioprotective adaptations caused by exercise. We hypothesize that part of the cardioprotective role of nNOS is via the augmentation of the antioxidant defense with exercise by positively shifting the nitroso-redox balance. Our results show that nNOS is indispensable for the augmented anti-oxidant defense with exercise. Furthermore, exercise training of nNOS knockout mice resulted in a negative shift in the nitroso-redox balance resulting in contractile dysfunction. Remarkably, overexpressing nNOS (conditional cardiac-specific nNOS overexpression) was able to mimic exercise by increasing VO2max. This study demonstrates that exercise results in a positive shift in the nitroso-redox balance that is nNOS-dependent. Thus, targeting nNOS signaling may mimic the beneficial effects of exercise by combating oxidative stress and may be a viable treatment strategy for heart disease.

KEYWORDS:

Exercise; Nitroso–redox balance; Phosphatase; Phospholamban; ROS

PMID:
25595735
PMCID:
PMC4380650
DOI:
10.1016/j.yjmcc.2015.01.003
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center