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J Cyst Fibros. 2015 Mar;14(2):237-41. doi: 10.1016/j.jcf.2014.12.014. Epub 2015 Jan 13.

Prevalence of Scedosporium species and Lomentospora prolificans in patients with cystic fibrosis in a multicenter trial by use of a selective medium.

Author information

1
Medizinische Hochschule Hannover, Medizinische Mikrobiologie und Krankenhaushygiene, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: Sedlacek.Ludwig@mh-hannover.de.
2
Labor Berlin - Charité Vivantes GmbH, Mikrobiologie/Mykologie, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: barbara.graf@laborberlin.com.
3
Christiane Herzog-Zentrum/Mukoviszidose, Klinik für Pädiatrie m.S. Pneumologie u. Immunologie, Sylter Str. 2, 13353 Berlin, Germany. Electronic address: carsten.schwarz@charite.de.
4
Universitätsklinikum Erlangen, Mikrobiologisches Institut, Wasserturmstraße 3-5, 91054 Erlangen, Germany. Electronic address: frauke.albert@labor-limbach-nuernberg.de.
5
Universitätsklinikum Tübingen, Institut für Medizinische Mikrobiologie und Hygiene, Elfriede-Aulhorn-Straße 6, 72076 Tübingen, Germany. Electronic address: silke.peter@med.uni-tuebingen.de.
6
Max von Pettenkofer-Institut, Medizinische Mikrobiologie, Pettenkoferstraße 9a, 80336 München, Germany. Electronic address: b.wuerstl@hygconcept.de.
7
Helios Klinik Emil von Behring, Institut für Mikrobiologie, Immunologie und Labormedizin, Walterhöferstraße 11, 14165 Berlin, Germany. Electronic address: sonja.wagner@helios-kliniken.de.
8
Universitätskliniken des Saarlandes, Institut für Med. Mikrobiologie und Hygiene, Kirrberger Straße, Gebäude 43, 66421 Homburg/Saar, Germany. Electronic address: michael.klotz@uks.eu.
9
Städtisches Klinikum Karlsruhe, Zentralinstitut für Laboratoriumsmedizin, Mikrobiologie und Transfusionsmedizin, Moltkestraße 90, 76133 Karlsruhe, Germany. Electronic address: andrea.becker@klinikum-karlsruhe.de.
10
RWTH Aachen, University Hospital, Institut für Med. Mikrobiologie, Pauwelsstraße 30, 52074 Aachen, Germany. Electronic address: ghaase@ukaachen.de.
11
Dr. v. Haunersches Kinderspital, Bakteriologisches Labor, Ludwig Maximilian Universität, Lindwurmstr. 4, 80337 München, Germany. Electronic address: gudrun.laniado@med.uni-muenchen.de.
12
Universitätsklinikum Münster, Institut für Med. Mikrobiologie, Domagkstraße 10, 48149 Münster, Germany. Electronic address: kahl@uni-muenster.de.
13
Medizinische Hochschule Hannover, Medizinische Mikrobiologie und Krankenhaushygiene, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: suerbaum.sebastian@mh-hannover.de.
14
Robert Koch-Institut, FG 16 Mykologie, Nordufer 20, 13353 Berlin, Germany. Electronic address: seiboldm@rki.de.
15
Robert Koch-Institut, FG 16 Mykologie, Nordufer 20, 13353 Berlin, Germany. Electronic address: tintelnotk@rki.de.

Abstract

OBJECTIVE:

Detection of hyphomycetes of the Scedosporium apiospermum complex and Lomentospora prolificans (Sac-Lp) is not yet standardized. Prevalence rates in patients with cystic fibrosis (CF) and the resistance pattern of these pathogens in Germany are unknown.

METHODS:

In a one-year prospective study 11 laboratories used a selective medium for isolation of Sac-Lp, examining >11,600 respiratory samples from 2346 patients with CF. Isolates were identified by molecular methods and tested for susceptibility to antifungal drugs.

RESULTS:

The prevalence of Sac-Lp in patients with CF in Germany varied from 0.0 to 10.5% (mean: 3.1%) among the clinical centres. The benefit of the selective medium SceSel(+) compared to standard media for fungi was documented for >5000 samples. High antifungal resistance was detected in the S. apiospermum complex, and the multiresistance of L. prolificans was confirmed.

CONCLUSION:

Microbiology laboratories should be aware of these resistant species in patients with CF and consider using a selective medium.

KEYWORDS:

Cystic fibrosis; Germany; Lomentospora; Prevalence; Scedosporium

PMID:
25595044
DOI:
10.1016/j.jcf.2014.12.014
[Indexed for MEDLINE]
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