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Nat Commun. 2015 Jan 16;6:6043. doi: 10.1038/ncomms7043.

A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants.

Author information

1
1] Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France [2] Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
2
1] Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France [2] Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux, CP 18534 Dakar, Senegal.
3
Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France.
4
Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France.
5
Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
6
Université Montpellier 2, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 34394 Montpellier, France.
7
Department of Plant Molecular Biology, Université de Lausanne, 1015 Lausanne, Switzerland.
8
Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.

Abstract

Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

PMID:
25592181
PMCID:
PMC4338584
DOI:
10.1038/ncomms7043
[Indexed for MEDLINE]
Free PMC Article

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