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Diagn Microbiol Infect Dis. 2015 Mar;81(3):153-7. doi: 10.1016/j.diagmicrobio.2014.10.009. Epub 2014 Oct 31.

Rapid detection of Gram-negative bacteria and their drug resistance genes from positive blood cultures using an automated microarray assay.

Author information

1
Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
2
Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: yjpk@catholic.ac.kr.

Abstract

We evaluated the performance of the Verigene Gram-negative blood culture (BC-GN) assay (CE-IVD version) for identification of Gram-negative (GN) bacteria and detection of resistance genes. A total of 163 GN organisms (72 characterized strains and 91 clinical isolates from 86 patients) were tested; among the clinical isolates, 86 (94.5%) isolates were included in the BC-GN panel. For identification, the agreement was 98.6% (146/148, 95% confidence interval [CI], 92.1-100) and 70% (7/10, 95% CI, 53.5-100) for monomicrobial and polymicrobial cultures, respectively. Of the 48 resistance genes harbored by 43 characterized strains, all were correctly detected. Of the 19 clinical isolates harboring resistance genes, 1 CTX-M-producing Escherichia coli isolated in polymicrobial culture was not detected. Overall, BC-GN assay provides acceptable accuracy for rapid identification of Gram-negative bacteria and detection of resistance genes, compared with routine laboratory methods despite that it has limitations in the number of genus/species and resistance gene included in the panel and it shows lower sensitivity in polymicrobial cultures.

KEYWORDS:

Automated microarray assay; Polymicrobial; Positive blood cultures; Verigene

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