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Int J Mol Sci. 2015 Jan 9;16(1):1562-75. doi: 10.3390/ijms16011562.

Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification.

Author information

1
Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan. just_dfm@hotmail.com.
2
Department & Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan. rich720925@yahoo.com.tw.
3
Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. linmk@mail.cmu.edu.tw.
4
Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. wtchang@mail.cmu.edu.tw.
5
Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. whpeng@mail.cmu.edu.tw.
6
Department of Bioresources, Da-Yeh University, Changhua 51591, Taiwan. fcsun@mail.dyu.edu.tw.
7
Management Center, Department of Medical Research and Development, Show Chwan Health Care Sysytem, Changhua 51951, Taiwan. mengshiunn@gmail.com.
8
Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. leemengshiou@mail.cmu.edu.tw.

Abstract

Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.

PMID:
25584616
PMCID:
PMC4307320
DOI:
10.3390/ijms16011562
[Indexed for MEDLINE]
Free PMC Article

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