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Infect Immun. 2015 Mar;83(3):1199-209. doi: 10.1128/IAI.02700-14. Epub 2015 Jan 12.

Polymyxin B resistance and biofilm formation in Vibrio cholerae are controlled by the response regulator CarR.

Author information

1
Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA.
2
Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA fyildiz@ucsc.edu.

Abstract

Two-component systems play important roles in the physiology of many bacterial pathogens. Vibrio cholerae's CarRS two-component regulatory system negatively regulates expression of vps (Vibrio polysaccharide) genes and biofilm formation. In this study, we report that CarR confers polymyxin B resistance by positively regulating expression of the almEFG genes, whose products are required for glycine and diglycine modification of lipid A. We determined that CarR directly binds to the regulatory region of the almEFG operon. Similarly to a carR mutant, strains lacking almE, almF, and almG exhibited enhanced polymyxin B sensitivity. We also observed that strains lacking almE or the almEFG operon have enhanced biofilm formation. Our results reveal that CarR regulates biofilm formation and antimicrobial peptide resistance in V. cholerae.

PMID:
25583523
PMCID:
PMC4333464
DOI:
10.1128/IAI.02700-14
[Indexed for MEDLINE]
Free PMC Article

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