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J Immunol. 2015 Feb 15;194(4):1677-85. doi: 10.4049/jimmunol.1402172. Epub 2015 Jan 12.

Heme exporter FLVCR is required for T cell development and peripheral survival.

Author information

1
Division of Hematology, University of Washington, Seattle, WA 98195; Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
2
Division of Hematology, University of Washington, Seattle, WA 98195;
3
Genomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA 98109;
4
University of Utah Metabolomics Core Facility, Salt Lake City, UT 84132; and.
5
Department of Immunology, University of Washington, Seattle, WA 98109.
6
Division of Hematology, University of Washington, Seattle, WA 98195; janabk@u.washington.edu.

Abstract

All aerobic cells and organisms must synthesize heme from the amino acid glycine and the tricarboxylic acid cycle intermediate succinyl CoA for incorporation into hemoproteins, such as the cytochromes needed for oxidative phosphorylation. Most studies on heme regulation have been done in erythroid cells or hepatocytes; however, much less is known about heme metabolism in other cell types. The feline leukemia virus subgroup C receptor (FLVCR) is a 12-transmembrane domain surface protein that exports heme from cells, and it was shown to be required for erythroid development. In this article, we show that deletion of Flvcr in murine hematopoietic precursors caused a complete block in αβ T cell development at the CD4(+)CD8(+) double-positive stage, although other lymphoid lineages were not affected. Moreover, FLVCR was required for the proliferation and survival of peripheral CD4(+) and CD8(+) T cells. These studies identify a novel and unexpected role for FLVCR, a major facilitator superfamily metabolite transporter, in T cell development and suggest that heme metabolism is particularly important in the T lineage.

PMID:
25582857
PMCID:
PMC4323866
DOI:
10.4049/jimmunol.1402172
[Indexed for MEDLINE]
Free PMC Article

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