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Sci Rep. 2015 Jan 13;5:7741. doi: 10.1038/srep07741.

Genomic and metabolic analysis of fluoranthene degradation pathway in Celeribacter indicus P73T.

Author information

1
1] State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China [2] School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
2
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China.

Abstract

Celeribacter indicus P73(T), isolated from deep-sea sediment from the Indian Ocean, is capable of degrading a wide range of polycyclic aromatic hydrocarbons (PAHs) and is the first fluoranthene-degrading bacterium within the family Rhodobacteraceae. Here, the complete genome sequence of strain P73(T) is presented and analyzed. Besides a 4.5-Mb circular chromosome, strain P73(T) carries five plasmids, and encodes 4827 predicted protein-coding sequences. One hundred and thirty-eight genes, including 14 dioxygenase genes, were predicted to be involved in the degradation of aromatic compounds, and most of these genes are clustered in four regions. P73_0346 is the first fluoranthene 7,8-dioxygenase to be discovered and the first fluoranthene dioxygenase within the toluene/biphenyl family. The degradative genes in regions B and D in P73(T) are absent in Celeribacter baekdonensis B30, which cannot degrade PAHs. Four intermediate metabolites [acenaphthylene-1(2H)-one, acenaphthenequinone, 1,2-dihydroxyacenaphthylene, and 1,8-naphthalic anhydride] of fluoranthene degradation by strain P73(T) were detected as the main intermediates, indicating that the degradation of fluoranthene in P73(T) was initiated by dioxygenation at the C-7,8 positions. Based on the genomic and metabolitic results, we propose a C-7,8 dioxygenation pathway in which fluoranthene is mineralized to TCA cycle intermediates.

PMID:
25582347
PMCID:
PMC4291564
DOI:
10.1038/srep07741
[Indexed for MEDLINE]
Free PMC Article

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