Comparison of cryopreservation bags for hematopoietic progenitor cells using a WBC-enriched product

Margriet J Dijkstra-Tiekstra; Sandra Hazelaar; Effimia Gkoumassi; Margienus Weggemans; Janny de Wildt-Eggen

doi:10.1016/j.transci.2014.12.022

Comparison of cryopreservation bags for hematopoietic progenitor cells using a WBC-enriched product

Transfus Apher Sci. 2015 Apr;52(2):187-93. doi: 10.1016/j.transci.2014.12.022. Epub 2014 Dec 23.

Authors

Margriet J Dijkstra-Tiekstra¹, Sandra Hazelaar², Effimia Gkoumassi², Margienus Weggemans³, Janny de Wildt-Eggen²

Affiliations

¹ Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands. Electronic address: margriet.j.dijkstra@gmail.com.
² Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands.
³ Sanquin Blood Supply, Division Blood Bank, Stem Cell Laboratory, Groningen, The Netherlands.

PMID: 25582270
DOI: 10.1016/j.transci.2014.12.022

Abstract

Hematopoietic progenitor cells (HPC) are stored in cryopreservation bags that are resistant to liquid nitrogen. Since Cryocyte bags of Baxter (B-bags) are no longer available, an alternative bag was sought. Also, the influence of freezing volume was studied. Miltenyi Biotec (MB)- and MacoPharma (MP)-bags passed the integrity tests without failure. Comparing MB- and MP-bags with B-bags, no difference in WBC recovery or viability was found when using a WBC-enriched product as a "dummy" HPC product. Further, a freezing volume of 30 mL resulted in better WBC recovery and viability than 60 mL. Additonal studies using real HPC might be necessary.

Keywords: Cryopreservation bags; Integrity; WBC recovery; WBC viability; WBC-enriched product.

Publication types

Comparative Study

MeSH terms

Blood Preservation / methods
Cell Survival
Cryopreservation / instrumentation*
Cryopreservation / methods*
Equipment Design
Freezing
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells / cytology*
Humans
Leukocyte Count
Leukocytes / cytology*
Specimen Handling