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Nat Methods. 2015 Mar;12(3):219-22, 5 p following 222. doi: 10.1038/nmeth.3250. Epub 2015 Jan 12.

Improved and expanded Q-system reagents for genetic manipulations.

Author information

1
The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
2
1] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [2] Department of Biology, Stanford University, Stanford, California, USA.
3
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Abstract

The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.

PMID:
25581800
PMCID:
PMC4344399
DOI:
10.1038/nmeth.3250
[Indexed for MEDLINE]
Free PMC Article

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