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Nat Methods. 2015 Mar;12(3):215-8, 4 p following 218. doi: 10.1038/nmeth.3225. Epub 2015 Jan 12.

Fixation-resistant photoactivatable fluorescent proteins for CLEM.

Author information

1
Howard Hughes Medical Institute, Janelia Farm Research Campus, Ashburn, Virginia, USA.
2
1] National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida, USA. [2] Department of Biological Science, Florida State University, Tallahassee, Florida, USA.
3
National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA.

Erratum in

  • Nat Methods. 2015 Jul;12(7):692.

Abstract

Fluorescent proteins facilitate a variety of imaging paradigms in live and fixed samples. However, they lose their fluorescence after heavy fixation, hindering applications such as correlative light and electron microscopy (CLEM). Here we report engineered variants of the photoconvertible Eos fluorescent protein that fluoresce and photoconvert normally in heavily fixed (0.5-1% OsO4), plastic resin-embedded samples, enabling correlative super-resolution fluorescence imaging and high-quality electron microscopy.

PMID:
25581799
PMCID:
PMC4344411
DOI:
10.1038/nmeth.3225
[Indexed for MEDLINE]
Free PMC Article

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