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Virology. 2015 Feb;476:316-322. doi: 10.1016/j.virol.2014.12.030. Epub 2015 Jan 9.

Antiviral effect of sulfated Chuanmingshen violaceum polysaccharide in chickens infected with virulent Newcastle disease virus.

Author information

1
(a)Natural Medecine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya׳an, 625014, China.
2
(a)Natural Medecine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya׳an, 625014, China. Electronic address: yinzhongq@163.com.
3
(a)Natural Medecine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Ya׳an, 625014, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya׳an, 625014, China. Electronic address: cqrc_jry@163.com.

Abstract

Newcastle disease virus (NDV) belonging to the Paramyxovirinae subfamily is one of the most devastating pathogens in poultry. Although vaccines are widely applied to control the infection, outbreaks of Newcastle disease (ND) repeatedly happen. Currently, there are no alternative control measures available for ND. In the present study, we found that sulfated Chuanmingshen violaceum polysaccharide (sCVPS) were potent inhibitors of NDV in specific pathogen free chickens infected with a virulent strain. With sCVPS treatment, the survival rate increased by almost 20% and virus titers in test organs, including brain, lung, spleen and thymus, were significantly decreased. The sCVPS also exhibited the ability to prevent viral transmission by reducing the amount of virus shed in saliva and feces. Higher concentrations of interferon α and γ in serum were detected in chickens treated with sCVPS, indicating that one of the antiviral mechanisms may be attributed to the property of immunoenhancement. Histopathological examination showed that sCVPS could alleviate the tissue lesions caused by NDV infection. These results suggest that sCVPS are expected to be a new alternative control measure for NDV infection and further studies could be carried out to evaluate the antiviral activity of sCVPS against other paramyxoviruses.

KEYWORDS:

Antiviral activity; Chuanmingshen violaceum polysaccharide; Newcastle disease virus; Sulfated polysaccharides

PMID:
25577148
DOI:
10.1016/j.virol.2014.12.030
[Indexed for MEDLINE]
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