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Toxicol In Vitro. 2015 Apr;29(3):479-88. doi: 10.1016/j.tiv.2014.12.022. Epub 2015 Jan 6.

Low concentrations of cylindrospermopsin induce increases of reactive oxygen species levels, metabolism and proliferation in human hepatoma cells (HepG2).

Author information

1
Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: samliebel@yahoo.com.br.
2
Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil.
3
Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP 21949-900, Rio de Janeiro, RJ, Brazil.
4
Departamento de Farmacologia, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil.
5
Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: filipak@ufpr.br.

Abstract

Human hepatoma cells (HepG2) were exposed to purified cylindrospermopsin (CYN), a potent toxicant for eukaryotic cells produced by several cyanobacteria. CYN was not toxic at concentrations up to 10 μgl(-1), leading to increased viability and metabolism in cells cultured with 10% fetal bovine serum (FBS). Reduction of FBS concentration to 2% and induction of cytochrome P450 (CYP) isoforms were performed in order to make xenobiotic-metabolizing capacity of HepG2 cells closest to that of 'normal' cells. HepG2 cells proliferated less after CYPs-induction, and this induction has lead to similar results of non-induced cells, except for few individual parameters such lipid peroxidation. Foremost, low concentrations of CYN (below or equal 10 μgl(-1)) have induced HepG2 cells proliferation and metabolism increase, which was not expected.

KEYWORDS:

CYPs; Cell lineage; Cyanotoxin; Cylindrospermopsin; HepG2

PMID:
25575781
DOI:
10.1016/j.tiv.2014.12.022
[Indexed for MEDLINE]

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