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Biomed Opt Express. 2014 Nov 12;5(12):4242-8. doi: 10.1364/BOE.5.004242. eCollection 2014 Dec 1.

Simultaneous optical coherence tomography and lipofuscin autofluorescence imaging of the retina with a single broadband light source at 480nm.

Author information

1
Engineering Research Center of Optical Instruments and Systems, Ministry of Education, Shanghai Key Lab of Modern Optical Systems, University of Shanghai for Science and Technology, No. 516 Jungong Road, Shanghai 200093, China ; Department of Biomedical Engineering, Florida International University, 10555 W Flagler Street, Miami, Florida, 33174, USA.
2
Department of Biomedical Engineering, Florida International University, 10555 W Flagler Street, Miami, Florida, 33174, USA.

Abstract

We accomplished spectral domain optical coherence tomography and auto-fluorescence microscopy for imaging the retina with a single broadband light source centered at 480 nm. This technique is able to provide simultaneous structural imaging and lipofuscin molecular contrast of the retina. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. To test the capabilities of the technique we periodically imaged the retinas of the same rats for four weeks. The images successfully demonstrated lipofuscin accumulation in the retinal pigment epithelium with aging. The experimental results showed that the dual-modal imaging system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.

KEYWORDS:

(110.4500) Optical coherence tomography; (170.2520) Fluorescence microscopy

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