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J Neuroinflammation. 2015 Jan 9;12:3. doi: 10.1186/s12974-014-0220-5.

Spermine reverses lipopolysaccharide-induced memory deficit in mice.

Author information

1
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. pam_fruhauf@yahoo.com.br.
2
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. rafaelineu@gmail.com.
3
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. ledianetomazi@gmail.com.
4
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. duartethiago89@yahoo.com.br.
5
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. cf.mello@smail.ufsm.br.
6
Department of Physiology and Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. cf.mello@smail.ufsm.br.
7
Graduation Program in Pharmacology, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil. maribel.rubin@gmail.com.
8
Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, Camobi, CEP: 97105900, Santa Maria, RS, Brazil. maribel.rubin@gmail.com.

Abstract

BACKGROUND:

Lipopolysaccharide (LPS) induces neuroinflammation and memory deficit. Since polyamines improve memory in various cognitive tasks, we hypothesized that spermine administration reverses LPS-induced memory deficits in an object recognition task in mice. The involvement of the polyamine binding site at the N-methyl-D-aspartate (NMDA) receptor and cytokine production in the promnesic effect of spermine were investigated.

METHODS:

Adult male mice were injected with LPS (250 μg/kg, intraperitoneally) and spermine (0.3 to 1 mg/kg, intraperitoneally) or ifenprodil (0.3 to 10 mg/kg, intraperitoneally), or both, and their memory function was evaluated using a novel object recognition task. In addition, cortical and hippocampal cytokines levels were measured by ELISA four hours after LPS injection.

RESULTS:

Spermine increased but ifenprodil decreased the recognition index in the novel object recognition task. Spermine, at doses that did not alter memory (0.3 mg/kg, intraperitoneally), reversed the cognitive impairment induced by LPS. Ifenprodil (0.3 mg/kg, intraperitoneally) reversed the protective effect of spermine against LPS-induced memory deficits. However, spermine failed to reverse the LPS-induced increase of cortical and hippocampal cytokine levels.

CONCLUSIONS:

Spermine protects against LPS-induced memory deficits in mice by a mechanism that involves GluN2B receptors.

PMID:
25573647
PMCID:
PMC4302583
DOI:
10.1186/s12974-014-0220-5
[Indexed for MEDLINE]
Free PMC Article

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