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Zhonghua Yi Xue Za Zhi. 2014 Nov 4;94(40):3163-6.

[Anti-tumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line in vitro].

[Article in Chinese]

Author information

1
Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
2
Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. Email: laoniulyb@163.com.

Abstract

OBJECTIVE:

To explore the antitumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line.

METHODS:

DDP-PLLA-CNTs were prepared with the method of ultrasound emulsification. The morphology of DDP-PLLA-CNTs was determined by scanning electron microscope (SEM). And its drug loading and drug release curve in vitro was detected by UV-Vis-NIR spectrophotometer. CCK8 was used to test the cytotoxic effects of DDP-PLLA-CNTs at different concentrations on QBC939 cell proliferation.Flow cytometry was employed to measure the changes of apoptotic rate.

RESULTS:

With excellent controlled-release characteristic of in vitro drug release, DDP-PLLA-CNTs inhibited the proliferation and significantly increased the apoptotic rate of QBC939 cell line.

CONCLUSION:

DDP-PLLA-CNTs have drug sustained-release characteristics and can significantly inhibit the proliferation of QBC939 cell line.

PMID:
25573313
[Indexed for MEDLINE]

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