Diagnosis of neuroinvasive astrovirus infection in an immunocompromised adult with encephalitis by unbiased next-generation sequencing

Clin Infect Dis. 2015 Mar 15;60(6):919-23. doi: 10.1093/cid/ciu912. Epub 2015 Jan 7.

Abstract

Metagenomic next-generation sequencing (NGS) was used to diagnose an unusual and fatal case of progressive encephalitis in an immunocompromised adult presenting at disease onset as bilateral hearing loss. The sequencing and confirmatory studies revealed neuroinvasive infection of the brain by an astrovirus belonging to a recently discovered VA/HMO clade.

Keywords: VA/HMO astrovirus clade; deafness; encephalitis; next-generation sequencing; pathogen discovery.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Astroviridae Infections / diagnosis*
  • Brain / pathology
  • Capsid Proteins / genetics
  • Encephalitis, Viral / complications
  • Encephalitis, Viral / diagnosis*
  • Fatal Outcome
  • Hearing Loss, Bilateral / etiology
  • High-Throughput Nucleotide Sequencing / methods
  • Humans
  • Leukemia, Lymphoid / complications
  • Mamastrovirus / genetics*
  • Mamastrovirus / isolation & purification*
  • Phylogeny

Substances

  • Capsid Proteins