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PLoS Genet. 2015 Jan 8;11(1):e1004931. doi: 10.1371/journal.pgen.1004931. eCollection 2015 Jan.

Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.

Author information

1
Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee, United Kingdom; Institut für Biologie, FG Mikrobiologie, Universität Kassel, Germany.
2
Institut für Biologie, FG Mikrobiologie, Universität Kassel, Germany.
3
Centre for Gene Regulation & Expression, College of Life Sciences, University of Dundee, Dundee, United Kingdom.
4
Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz, Germany.
5
Institut für Biologie, FG Mikrobiologie, Universität Kassel, Germany; Department of Genetics, University of Leicester, Leicester, United Kingdom.

Abstract

Elongator is a conserved protein complex comprising six different polypeptides that has been ascribed a wide range of functions, but which is now known to be required for modification of uridine residues in the wobble position of a subset of tRNAs in yeast, plants, worms and mammals. In previous work, we showed that Elongator's largest subunit (Elp1; also known as Iki3) was phosphorylated and implicated the yeast casein kinase I Hrr25 in Elongator function. Here we report identification of nine in vivo phosphorylation sites within Elp1 and show that four of these, clustered close to the Elp1 C-terminus and adjacent to a region that binds tRNA, are important for Elongator's tRNA modification function. Hrr25 protein kinase directly modifies Elp1 on two sites (Ser-1198 and Ser-1202) and through analyzing non-phosphorylatable (alanine) and acidic, phosphomimic substitutions at Ser-1198, Ser-1202 and Ser-1209, we provide evidence that phosphorylation plays a positive role in the tRNA modification function of Elongator and may regulate the interaction of Elongator both with its accessory protein Kti12 and with Hrr25 kinase.

PMID:
25569479
PMCID:
PMC4287497
DOI:
10.1371/journal.pgen.1004931
[Indexed for MEDLINE]
Free PMC Article

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