Send to

Choose Destination
Ultrastruct Pathol. 2015 Apr;39(2):79-87. doi: 10.3109/01913123.2014.980482. Epub 2015 Jan 8.

On the maturation of megakaryocytes: a review with original observations on human in vivo cells emphasizing morphology and ultrastructure.

Author information

State Key Laboratory of Experimental Hematology, Peking Union Medical College, Institute of Hematology & Blood Diseases Hospital , Beijing , China and.


Megakaryocytes engage in the synthesis of a variety of molecular and macromolecular constituents to build-up characteristic megakaryocyte structure and form proplatelets in a series of cells from megakaryocyte precursors to the fully matured cell. The process is illustrated in this review by light microscope morphology and transmission electron microscopy, which emphasizes new findings in human in vivo megakaryocytes, thereby making a contrast with the abundant literature on megakaryocytes from experimental animal and human in vitro material. Four stages are identified and described, based on the development of characteristic structures including α-granules, dense granules (dense-core granules), the demarcation membrane system (DMS), and proplatelets. The mechanism of DMS development is discussed, in terms of hypotheses suggesting origin from the plasma membrane, and contributions of membrane from the Golgi apparatus and endoplasmic reticulum. The formation of the marginal zone is also discussed, which is suggested to result from a circumscription of the peripheral organelle-free cytoplasmic fringe by peripheral circular cytoskeletal elements such as cytoplasmic actin and microtubules.


Development; megakaryocytes; transmission electron microscopy; ultrastructure

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center