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J Virol Methods. 1989 Oct;26(1):27-33.

A plaque hybridization assay for rotaviruses.

Author information

1
Station de Virologie et d'Immunologie Mol├ęculaires, INRA, Jouy-en-Josas, France.

Abstract

A plaque hybridization assay was adapted to rotavirus. In this test cDNA or oligonucleotide probes were used to discriminate between plaques originating from virus carrying genes of bovine and simian origin. Only mRNAs present in infected cells were detected as demonstrated by using oligonucleotides corresponding to both strands. This assay can be used to screen reassortants or mutants of rotaviruses.

PMID:
2556423
DOI:
10.1016/0166-0934(89)90071-2
[Indexed for MEDLINE]

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