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Curr Protoc Mol Biol. 2015 Jan 5;109:21.29.1-9. doi: 10.1002/0471142727.mb2129s109.

ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.

Author information

1
Department of Genetics, Stanford University School of Medicine, Stanford, California; Program in Epithelial Biology and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California.

Abstract

This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription-factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assaying nucleosome positions in accessible regions of the genome.

KEYWORDS:

ATAC-seq; chromatin accessibility; transposase

PMID:
25559105
PMCID:
PMC4374986
DOI:
10.1002/0471142727.mb2129s109
[Indexed for MEDLINE]
Free PMC Article

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