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Chem Biol. 2015 Jan 22;22(1):139-47. doi: 10.1016/j.chembiol.2014.11.011. Epub 2014 Dec 31.

Probes to monitor activity of the paracaspase MALT1.

Author information

1
Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA; Graduate School of Biomedical Sciences, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA.
2
Cancer Biology Program, Stanford School of Medicine, Stanford, CA 94305, USA; Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, USA.
3
Division of Bioorganic Chemistry, Wroclaw University of Technology, 50-370 Wroclaw, Poland.
4
Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford School of Medicine, Stanford, CA 94305, USA.
5
Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, USA; Department of Microbiology and Immunology, Stanford School of Medicine, Stanford, CA 94305, USA.
6
Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA. Electronic address: gsalvesen@sanfordburnham.org.

Abstract

The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolytic activity. The probes selectively label active enzyme and can be used to inhibit MALT1 and trace its activity profile, helping to create a better picture of the significance of the proteolytic function of MALT1.

PMID:
25556944
PMCID:
PMC4304901
DOI:
10.1016/j.chembiol.2014.11.011
[Indexed for MEDLINE]
Free PMC Article

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