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Neuron. 2015 Jan 21;85(2):275-288. doi: 10.1016/j.neuron.2014.12.024. Epub 2014 Dec 31.

DeCoN: genome-wide analysis of in vivo transcriptional dynamics during pyramidal neuron fate selection in neocortex.

Author information

1
Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, United States.
2
Broad Institute of MIT and Harvard, Cambridge, MA, 02139, United States.
3
Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, 02139, United States.
4
Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02115, United States.
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Contributed equally

Erratum in

  • Neuron. 2016 Jan 6;89(1):235.

Abstract

Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long noncoding RNAs with restricted temporal and cell-type-specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multifaceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.

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PMID:
25556833
PMCID:
PMC4430475
DOI:
10.1016/j.neuron.2014.12.024
[Indexed for MEDLINE]
Free PMC Article

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