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Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2014 Dec;36(6):614-9. doi: 10.3881/j.issn.1000-503X.2014.06.010.

Effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in mice.

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Department of Endocrinology,the Second Xiangya Hospital,Central South University, Diabetes Center,Institute of Metabolism and Endocrinology,Central South University,Diabetes Immunology Ministry of Education Key Laboratory,National Clinical Research Center for Metabolic Disease,Changsha 410011,China.



To investigate the effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in C57BL/6 mice.


Four-week-old C57BL/6 mice were allocated into normal diet group,high-fat diet group,and high-glucose diet group according to the random number table until 20 weeks old. Body weight,epididymal adipose tissue weight,blood leptin,fat infiltration in liver,M1/M2 macrophage subtypes,and monocyte chemoattractant protein-1 mRNA in epididymal adipose tissues were measured.


Compared with normal diet group,body weight,epididymal adipose tissue weight,and leptin concentration in high fat diet group at 20 weeks were significantly increased (P < 0.05),and oil red O staining showed more prominent adipocyte infiltration in liver in high-fat diet group than those in normal diet and high-glucose diet group. However,no apparent differences were seen in high-glucose diet group at 20 weeks in terms of body weight,epididymal adipose tissue weight and leptin concentration. In high-fat diet group,the macrophages infiltration in epididymal adipose tissue increased with time and the percentage of M2 macrophage decreased in high-fat diet group than that in high-glucose diet group(P<0.05). Compared with normal diet group,monocyte chemoattractant protein-1 mRNA expression increased significantly in high-fat diet group(P<0.05). In high-glucose group,however,no significant differences were discerned (P > 0.05).


High-fat diet,rather than 60% high glucose diet,will lead to obesity and macrophage infiltration in adipose tissues.

[Indexed for MEDLINE]
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