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Asian Pac J Cancer Prev. 2014;15(23):10115-9.

IGF-1 from adipose-derived mesenchymal stem cells promotes radioresistance of breast cancer cells.

Author information

1
Department of Anatomy, Nanfang Hospital, Southern Medical University, Guangzhou, China E-mail : daijingxing@gmail.com; ylsmuanatomy@163.com.

Abstract

PURPOSE:

The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells.

MATERIALS AND METHODS:

MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; γ-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting.

RESULTS:

AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells.

CONCLUSIONS:

Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.

PMID:
25556435
DOI:
10.7314/apjcp.2014.15.23.10115
[Indexed for MEDLINE]
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