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Neurotoxicology. 2015 Jan;46:125-36. doi: 10.1016/j.neuro.2014.12.010. Epub 2014 Dec 31.

The effect of the aquatic contaminants bisphenol-A and PCB-95 on the zebrafish lateral line.

Author information

1
College of Arts and Sciences, Washington State University, Vancouver, WA, USA. Electronic address: lauren.hayashi@wsu.edu.
2
College of Arts and Sciences, Washington State University, Vancouver, WA, USA. Electronic address: meghal.sheth23@gmail.com.
3
College of Arts and Sciences, Washington State University, Vancouver, WA, USA. Electronic address: a.young1204@gmail.com.
4
College of Arts and Sciences, Washington State University, Vancouver, WA, USA. Electronic address: matthew.kruger@email.wsu.edu.
5
Department of Integrative Physiology and Neuroscience, Washington State University, Pullman, WA, USA. Electronic address: waymang@vetmed.wsu.edu.
6
College of Arts and Sciences, Washington State University, Vancouver, WA, USA; Department of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA, USA. Electronic address: Allison.coffin@wsu.edu.

Abstract

Environmental toxicants such as bisphenol-A (BPA) and polychlorinated biphenyls (PCBs) are prevalent in our water supply, soil, and many food products and can profoundly affect the central nervous system. Both BPA and PCBs can disrupt endocrine signaling, which is important for auditory development and function, but the effect of these toxicants on the auditory periphery is not understood. In this study we investigated the effect of PCB-95 and BPA on lateral line development, function, and regeneration in larval zebrafish. The lateral line is a system of mechanosensory hair cells on the exterior of the fish that are homologous to the hair cells located in the mammalian inner ear. We found that PCB-95 had no effect on lateral line development or hair cell survival. BPA also did not affect lateral line development, but instead had a significant effect on both hair cell survival and regeneration. BPA-induced hair cell loss is both dose- and time-dependent, with concentrations of 1 μM or higher killing lateral line hair cells during a 24h exposure period. Pharmacologic manipulation experiments suggest that BPA kills hair cells via activation of oxidative stress pathways, similar to prior reports of BPA toxicity in other tissues. We also observed that hair cells killed with neomycin, a known ototoxin, failed to regenerate normally when BPA was present, suggesting that BPA in aquatic environments could impede innate regenerative responses in fishes. Collectively, these data demonstrate that BPA can have detrimental effects on sensory systems, both in aquatic life and perhaps in terrestrial organisms, including humans.

KEYWORDS:

Bisphenol-A; Hair cell; Hearing; Lateral line; Polychlorinated biphenyl

PMID:
25556122
PMCID:
PMC5973882
DOI:
10.1016/j.neuro.2014.12.010
[Indexed for MEDLINE]
Free PMC Article

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