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Int J Biol Macromol. 2015 Mar;74:337-42. doi: 10.1016/j.ijbiomac.2014.12.007. Epub 2014 Dec 30.

Folic acid binds DNA and RNA at different locations.

Author information

1
Department of Chemistry-Biochemistry-Physics, University of Québec at Trois-Rivières, C.P. 500, Trois-Rivières, Québec G9A 5H7, Canada.
2
Department of Chemistry-Biochemistry-Physics, University of Québec at Trois-Rivières, C.P. 500, Trois-Rivières, Québec G9A 5H7, Canada. Electronic address: tajmirri@uqtr.ca.

Abstract

We located multiple binding sites for folic acid on DNA and tRNA at physiological conditions, using FTIR, CD, fluorescence spectroscopic methods and molecular modeling. Structural analysis revealed that folic acid binds DNA and tRNA at multiple sites via hydrophilic, hydrophobic and H-bonding contacts with overall binding constants of Kfolic acid-DNA=1.1 (±0.3)×10(4) M(-1) and Kfolic acid-tRNA=6.4 (±0.5)×10(3) M(-1). Molecular modeling showed the participation of several nucleobases in folic acid complexes with DNA and tRNA, stabilized by H-bonding network. Two types of complexes were located for folic acid-tRNA adducts, one at the major groove and the other with TΨC loop, while acid binding occurs at major and minor grooves of DNA duplex. Folic acid complexation induced more alterations of DNA structure than tRNA.

KEYWORDS:

Binding site; DNA; Folic acid; Modeling; Spectroscopy; tRNA

PMID:
25555838
DOI:
10.1016/j.ijbiomac.2014.12.007
[Indexed for MEDLINE]
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