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Osteoarthritis Cartilage. 2015 Apr;23(4):640-7. doi: 10.1016/j.joca.2014.12.017. Epub 2014 Dec 30.

Cartilage boundary lubrication of ovine synovial fluid following anterior cruciate ligament transection: a longitudinal study.

Author information

1
McCaig Institute for Bone and Joint Health, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: matarodp@ucalgary.ca.
2
Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada. Electronic address: teludwig@ucalgary.ca.
3
Department of Surgery, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada. Electronic address: cfrank@ucalgary.ca.
4
Faculty of Kinesiology, Biomedical Engineering, University of Calgary, Calgary, Alberta, Canada. Electronic address: tschmidt@ucalgary.ca.
5
Department of Civil Engineering, Schulich School of Engineering, University of Calgary, Calgary, Alberta, Canada. Electronic address: ngshrive@ucalgary.ca.

Abstract

OBJECTIVE:

To assess ovine synovial fluid (oSF) from different post-injury time points for (1) proteoglycan-4 (PRG4) and hyaluronan (HA) concentration, (2) HA molecular weight (MW) distribution, (3) cartilage boundary lubrication function, and (4) lubricant composition-function relationships. The association between cartilage boundary lubrication and gross cartilage changes after injury was also examined.

METHODS:

oSF was collected 2, 4, 10, and 20 weeks post anterior cruciate ligament (ACL) transection in five skeletally mature sheep. PRG4 and HA concentrations were measured using sandwich enzyme-linked immunosorbent assay, and HA MW distribution by agarose gel electrophoresis. Cartilage boundary lubrication of oSF was assessed using a cartilage-cartilage friction test. Gross damage to articular cartilage was also quantified at 20 weeks using modified Drez scoring protocol.

RESULTS:

Early (2-4 weeks) after ACL injury, PRG4 concentrations were significantly higher (P = 0.045, P = 0.037), and HA concentrations were substantially lower (P = 0.005, P = 0.005) compared to 20 weeks. The HA MW distribution also shifted towards lower ranges in the early post-injury stage. The kinetic friction coefficients were significantly higher 2-4 weeks post injury (P = 0.008 and P = 0.049) compared to 20 weeks. Poor cartilage boundary lubricating ability early after injury was associated with cartilage damage at 20 weeks.

CONCLUSION:

Altered composition and diminished boundary lubrication of oSF early after ACL transection may pre-dispose the articular cartilage to degenerative changes and initiate osteoarthritis (OA). These observations also provide potential motivation for biotherapeutic interventions at earlier time points post injury.

KEYWORDS:

ACL injury; Boundary lubrication; Cartilage; Ovine; Synovial fluid

PMID:
25554643
DOI:
10.1016/j.joca.2014.12.017
[Indexed for MEDLINE]
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