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Front Biosci (Landmark Ed). 2015 Jan 1;20:139-56.

Isoflurane attenuates LPS-induced acute lung injury by targeting miR-155-HIF1-alpha.

Author information

1
Department of Anesthesiology, Shanghai Ninth People�s Hospital affiliated to Shanghai Jiao Tong University, School of Medicine, No. 639, Zhi Zao Ju Road, Shanghai, China.

Abstract

Isoflurane alleviates the inflammatory response in endotoxin-induced acute lung injury (ALI). In this study, we investigated the protective mechanism of isoflurane postconditioning in lipopolysaccharide (LPS)induced ALI. Exposure to isoflurane decreased miR-155 and upregulated HIF-1 alpha and HO-1 mRNA and protein. The effects of isoflurane on HIF-1 alpha mRNA and protein could be inhibited by overexpression of miR-155. Furthermore, mice overexpressing miR-155 had higher levels of TNF-alpha and IL-1 beta in BALF when exposed to isoflurane after LPS challenge.Conversely, downregulation of miR-155 promoted isoflurane effects on HIF-1 alpha expression. These results suggest that isoflurane posttreatment hr alleviates LPS-induced ALI and cell injury by triggering miR-155-HIF-1 alpha pathway, leading to upregulation of HO-1.

PMID:
25553444
DOI:
10.2741/4302
[Indexed for MEDLINE]

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