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G3 (Bethesda). 2014 Dec 31;5(3):361-70. doi: 10.1534/g3.114.015701.

Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.

Author information

1
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
2
Department of Genetics, Evolution and Environment and UCL Cancer Institute, University College London, London WC1E 6BT, United Kingdom.
3
Genome Technology Center, Stanford University School of Medicine, Palo Alto, California 01605.
4
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
5
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 kathy.gould@vanderbilt.edu.

Abstract

Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.

KEYWORDS:

DNA metabolism; cell division; fission yeast; gene deletion; stress sensitivity

PMID:
25552606
PMCID:
PMC4349090
DOI:
10.1534/g3.114.015701
[Indexed for MEDLINE]
Free PMC Article

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