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PLoS One. 2014 Dec 31;9(12):e115817. doi: 10.1371/journal.pone.0115817. eCollection 2014.

Olive oil and vitamin D synergistically prevent bone loss in mice.

Author information

1
Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche (UMR) 1019, Unité de Nutrition Humaine, Centre de Recherche en Nutrition Humaine (CRNH) Auvergne, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Clermont-Ferrand, France; Lesieur, 29 quai Aulagnier, 92665 Asnières-sur-Seine cedex, France.
2
Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche (UMR) 1019, Unité de Nutrition Humaine, Centre de Recherche en Nutrition Humaine (CRNH) Auvergne, Clermont-Ferrand, France; Clermont Université, Université d'Auvergne, Clermont-Ferrand, France.
3
Biochemistry Department, Centre Technique de Conservation des Produits Agricoles (CTCPA), Site Agroparc, 84911 Avignon Cedex 9, France.
4
INRA, UMR 1260, Marseille, France; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1260, "Nutrition, Obésité et Risque Thrombotique", Marseille, France; Université d'Aix-Marseille, Faculté de Médecine, Marseille, France.
5
Lesieur, 29 quai Aulagnier, 92665 Asnières-sur-Seine cedex, France.

Abstract

As the Mediterranean diet (and particularly olive oil) has been associated with bone health, we investigated the impact of extra virgin oil as a source of polyphenols on bone metabolism. In that purpose sham-operated (SH) or ovariectomized (OVX) mice were subjected to refined or virgin olive oil. Two supplementary OVX groups were given either refined or virgin olive oil fortified with vitamin D3, to assess the possible synergistic effects with another liposoluble nutrient. After 30 days of exposure, bone mineral density and gene expression were evaluated. Consistent with previous data, ovariectomy was associated with increased bone turnover and led to impaired bone mass and micro-architecture. The expression of oxidative stress markers were enhanced as well. Virgin olive oil fortified with vitamin D3 prevented such changes in terms of both bone remodeling and bone mineral density. The expression of inflammation and oxidative stress mRNA was also lower in this group. Overall, our data suggest a protective impact of virgin olive oil as a source of polyphenols in addition to vitamin D3 on bone metabolism through improvement of oxidative stress and inflammation.

PMID:
25551374
PMCID:
PMC4281074
DOI:
10.1371/journal.pone.0115817
[Indexed for MEDLINE]
Free PMC Article

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