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J Vis Exp. 2014 Dec 12;(94). doi: 10.3791/52248.

Recovery of adult zebrafish hearts for high-throughput applications.

Author information

1
Cardiovascular Research Institute and Division of Cardiology, University of California San Francisco; Rima.Arnaout@ucsf.edu.
2
Department for Biochemistry and Biophysics, University of California San Francisco; Max-Planck Institute for Heart and Lung Research.

Abstract

Use of the zebrafish model system for studying development, regeneration, and disease is expanding toward use of adult hearts for cell dissociation and purification of RNA, DNA, and proteins. All of these applications demand the rapid recovery of significant numbers of zebrafish hearts to avoid gene regulatory, metabolic, and other changes that begin after death. Adult zebrafish hearts are also required for studying heart structure for a variety of mutants and for studying heart regeneration. However, the traditional zebrafish heart dissection is slow and difficult and requires specialized tools, making large-scale dissection of adult zebrafish hearts tedious. Traditional methods also harbor the risk of damaging the heart during the dissection. Here, we describe a method for dissection of adult zebrafish hearts that is fast, reproducible, and preserves heart architecture. Furthermore, this method does not require specialized tools, is painless for the zebrafish, can be performed on fresh or fixed specimens, and can be performed on zebrafish as young as one month old. The approach described expands the use of adult zebrafish for cardiovascular research.

PMID:
25548868
PMCID:
PMC4396955
DOI:
10.3791/52248
[Indexed for MEDLINE]
Free PMC Article

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