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Mol Cell. 2015 Jan 8;57(1):55-68. doi: 10.1016/j.molcel.2014.11.019. Epub 2014 Dec 24.

Phosphorylation of LC3 by the Hippo kinases STK3/STK4 is essential for autophagy.

Author information

  • 1Sanford-Burnham Medical Research Institute, Development, Aging, and Regeneration Program, La Jolla, CA 92037, USA.
  • 2Department of Pediatrics, School of Medicine and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • 3Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
  • 4Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
  • 5The Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
  • 6Sanford-Burnham Medical Research Institute, Development, Aging, and Regeneration Program, La Jolla, CA 92037, USA. Electronic address: mhansen@sanfordburnham.org.

Abstract

The protein LC3 is indispensible for the cellular recycling process of autophagy and plays critical roles during cargo recruitment, autophagosome biogenesis, and completion. Here, we report that LC3 is phosphorylated at threonine 50 (Thr(50)) by the mammalian Sterile-20 kinases STK3 and STK4. Loss of phosphorylation at this site blocks autophagy by impairing fusion of autophagosomes with lysosomes, and compromises the ability of cells to clear intracellular bacteria, an established cargo for autophagy. Strikingly, mutation of LC3 mimicking constitutive phosphorylation at Thr(50) reverses the autophagy block in STK3/STK4-deficient cells and restores their capacity to clear bacteria. Loss of STK3/STK4 impairs autophagy in diverse species, indicating that these kinases are conserved autophagy regulators. We conclude that phosphorylation of LC3 by STK3/STK4 is an essential step in the autophagy process. Since several pathological conditions, including bacterial infections, display aberrant autophagy, we propose that pharmacological agents targeting this regulatory circuit hold therapeutic potential.

PMID:
25544559
PMCID:
PMC4373083
DOI:
10.1016/j.molcel.2014.11.019
[PubMed - indexed for MEDLINE]
Free PMC Article

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