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Int J Infect Dis. 2015 Apr;33:185-90. doi: 10.1016/j.ijid.2014.12.038. Epub 2014 Dec 25.

Vancomycin intermediate-resistant Staphylococcus aureus (VISA) isolated from a patient who never received vancomycin treatment.

Author information

1
Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2
Department of Clinical Laboratory, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
3
Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. Electronic address: tjszyong@163.com.

Abstract

BACKGROUND:

With the abuse of antibiotics, the methicillin-resistant Staphylococcus aureus (MRSA) strain became prevalent. Furthermore, Staphylococcus aureus with a character of vancomycin intermediate-resistance (VISA) has been found globally since the first report in Japan. The main objectives of this study were to report a case of VISA isolated from a Chinese patient who had never undergone Vancomycin treatment, and to determine its molecular character.

METHODS:

A total of 9 strains were recovered from a patient during the therapeutic process. Antimicrobial susceptibility testing was performed to determine their antibiotic susceptibility patterns. To detect the VISA strain's molecular epidemiological features, growth and morphological characters, we used multilocus sequence typing, autolysis assay and transmission electric microscope tests. Pulsed-field gel electrophoresis (PFGE) was performed to characterize the heterogeneities of all isolates.

RESULTS:

One isolate was found to exhibit vancomycin intermediated-resistant with MIC of 8 μg/ml. It was ST239-T030-agr-1, had thickened cell wall, and displayed a slower growth rate and reduced susceptibility to Triton X-100-induced autolysis than other strains. All 9 strains exhibited the same PFGE pattern.

CONCLUSION:

This is the first report of VISA found in central China from a patient who had never received vancomycin treatment.

KEYWORDS:

Molecular typing; Staphylococcus; VISA; Vancomycin intermediated-resistant Staphylococcus aureus

PMID:
25543098
DOI:
10.1016/j.ijid.2014.12.038
[Indexed for MEDLINE]
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