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Anal Biochem. 2015 Mar 15;473:63-5. doi: 10.1016/j.ab.2014.12.007. Epub 2014 Dec 24.

Automatic cell counting with ImageJ.

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Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA 90089, USA. Electronic address:


Cell counting is an important routine procedure. However, to date there is no comprehensive, easy to use, and inexpensive solution for routine cell counting, and this procedure usually needs to be performed manually. Here, we report a complete solution for automatic cell counting in which a conventional light microscope is equipped with a web camera to obtain images of a suspension of mammalian cells in a hemocytometer assembly. Based on the ImageJ toolbox, we devised two algorithms to automatically count these cells. This approach is approximately 10 times faster and yields more reliable and consistent results compared with manual counting.


Cell counting; ImageJ; Macro; Microscope camera

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