Format

Send to

Choose Destination
PLoS One. 2014 Dec 26;9(12):e111758. doi: 10.1371/journal.pone.0111758. eCollection 2014.

Mechanical stress downregulates MHC class I expression on human cancer cell membrane.

Author information

1
Department of Experimental and Clinical Medicine, University of "Magna Graecia", Catanzaro, Italy; Italian Institute of Technology (IIT), Genova, Italy.
2
Department of Experimental and Clinical Medicine, University of "Magna Graecia", Catanzaro, Italy.
3
Department of Experimental and Clinical Medicine, University of "Magna Graecia", Catanzaro, Italy; Department of Microbiology, College of Medicine, University of Thi-Qar, Nasseriah, Iraq.
4
Department of Experimental and Clinical Medicine, University of "Magna Graecia", Catanzaro, Italy; King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.
5
Science for Life Laboratory, Department of Medicine, Karolinska Institute, Stockholm, Sweden.
6
Nanotech Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark.
7
Department of Surgical and Medical Sciences, University of "Magna Graecia", Catanzaro, Italy.
8
Endocrinology and Experimental Oncology Institute, CNR, Napoli, Italy.
9
Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.
10
Department of Experimental and Clinical Medicine, University of "Magna Graecia", Catanzaro, Italy; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.

Abstract

In our body, cells are continuously exposed to physical forces that can regulate different cell functions such as cell proliferation, differentiation and death. In this work, we employed two different strategies to mechanically stress cancer cells. The cancer and healthy cell populations were treated either with mechanical stress delivered by a micropump (fabricated by deep X-ray nanolithography) or by ultrasound wave stimuli. A specific down-regulation of Major Histocompatibility Complex (MHC) class I molecules expression on cancer cell membrane compared to different kinds of healthy cells (fibroblasts, macrophages, dendritic and lymphocyte cells) was observed, stimulating the cells with forces in the range of nano-newton, and pressures between 1 and 10 bar (1 bar = 100.000 Pascal), depending on the devices used. Moreover, Raman spectroscopy analysis, after mechanical treatment, in the range between 700-1800 cm(-1), indicated a relative concentration variation of MHC class I. PCA analysis was also performed to distinguish control and stressed cells within different cell lines. These mechanical induced phenotypic changes increase the tumor immunogenicity, as revealed by the related increased susceptibility to Natural Killer (NK) cells cytotoxic recognition.

PMID:
25541692
PMCID:
PMC4277281
DOI:
10.1371/journal.pone.0111758
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center