Format

Send to

Choose Destination
J Exp Bot. 2015 Mar;66(5):1339-53. doi: 10.1093/jxb/eru484. Epub 2014 Dec 24.

AtDsPTP1 acts as a negative regulator in osmotic stress signalling during Arabidopsis seed germination and seedling establishment.

Author information

1
College of Life Science, Shandong University, Jinan, Shandong, China Department of Biology, Hong Kong Baptist University, Hong Kong, China.
2
State Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Taian, Shandong, China.
3
Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China.
4
College of Life Sciences, South China Agricultural University, Guangdong, China.
5
School of Life Science and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.
6
Department of Biology, Hong Kong Baptist University, Hong Kong, China.
7
College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
8
Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China School of Life Science and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China jhzhang@cuhk.edu.hk.

Abstract

Dual-specificity protein phosphatases (DsPTPs) target both tyrosine and serine/threonine residues and play roles in plant growth and development. We have characterized an Arabidopsis mutant, dsptp1, which shows a higher seed germination rate and better root elongation under osmotic stress than the wild type. By contrast, its overexpression line, DsPTP1-OE, shows inhibited seed germination and root elongation; and its complemented line, DsPTP1-Com, resembles the wild type and rescues DsPTP1-OE under osmotic stress. Expression of AtDsPTP1 is enhanced by osmotic stress in seed coats, bases of rosette leaves, and roots. Compared with the wild type, the dsptp1 mutant shows increased proline accumulation, reduced malondialdehyde (MDA) content and ion leakage, and enhanced antioxidant enzyme activity in response to osmotic stress. AtDsPTP1 regulates the transcript levels of various dehydration-responsive genes under osmotic stress. Abscisic acid (ABA) accumulation in dsptp1 under osmotic stress is reduced with reduced expression of the ABA-biosynthesis gene NCED3 and increased expression of the ABA-catabolism gene CYP707A4. AtDsPTP1 also regulates the expression of key components in the ABA-signalling pathway. In conclusion, AtDsPTP1 regulates ABA accumulation, and acts as a negative regulator in osmotic stress signalling during Arabidospsis seed germination and seedling establishment.

KEYWORDS:

Abscisic acid (ABA); Arabidopsis; dual-specificity protein phosphatase; osmotic stress; root elongation; seed germination.

PMID:
25540435
PMCID:
PMC4339596
DOI:
10.1093/jxb/eru484
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center