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BMC Syst Biol. 2014 Dec 24;8:139. doi: 10.1186/s12918-014-0139-6.

Invariance and plasticity in the Drosophila melanogaster metabolomic network in response to temperature.

Author information

1
Department of Pathology, University of Washington, Box 357705, Seattle, WA, 98195, USA. rmhariharan123@gmail.com.
2
Laboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, 230-0045, Japan. rmhariharan123@gmail.com.
3
Department of Genetics, University of Georgia, Athens, GA, 30602, USA. jmhoffm@uga.edu.
4
Department of Genetics, University of Georgia, Athens, GA, 30602, USA. thomasa@wusm.wustl.edu.
5
Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO, 63108, USA. thomasa@wusm.wustl.edu.
6
Division of Pulmonary Allergy & Critical Care Medicine, Emory University, Atlanta, GA, 30322, USA. quinlyn.soltow@clinmet.com.
7
Department of Medicine, Clinical Biomarkers Laboratory, Emory University, Atlanta, GA, 30322, USA. quinlyn.soltow@clinmet.com.
8
ClinMet Inc, 3210 Merryfield Row, San Diego, CA, 92121, USA. quinlyn.soltow@clinmet.com.
9
Division of Pulmonary Allergy & Critical Care Medicine, Emory University, Atlanta, GA, 30322, USA. dpjones@emory.edu.
10
Department of Medicine, Clinical Biomarkers Laboratory, Emory University, Atlanta, GA, 30322, USA. dpjones@emory.edu.
11
Department of Pathology, University of Washington, Box 357705, Seattle, WA, 98195, USA. promislo@uw.edu.
12
Department of Biology, University of Washington, Seattle, WA, 98195, USA. promislo@uw.edu.

Abstract

BACKGROUND:

Metabolomic responses to extreme thermal stress have recently been investigated in Drosophila melanogaster. However, a network level understanding of metabolomic responses to longer and less drastic temperature changes, which more closely reflect variation in natural ambient temperatures experienced during development and adulthood, is currently lacking. Here we use high-resolution, non-targeted metabolomics to dissect metabolomic changes in D. melanogaster elicited by moderately cool (18°C) or warm (27°C) developmental and adult temperature exposures.

RESULTS:

We find that temperature at which larvae are reared has a dramatic effect on metabolomic network structure measured in adults. Using network analysis, we are able to identify modules that are highly differentially expressed in response to changing developmental temperature, as well as modules whose correlation structure is strongly preserved across temperature.

CONCLUSIONS:

Our results suggest that the effect of temperature on the metabolome provides an easily studied and powerful model for understanding the forces that influence invariance and plasticity in biological networks.

PMID:
25540032
PMCID:
PMC4302152
DOI:
10.1186/s12918-014-0139-6
[Indexed for MEDLINE]
Free PMC Article

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