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Nucleic Acids Res. 2015 Jan;43(2):775-86. doi: 10.1093/nar/gku1346. Epub 2014 Dec 24.

Incorporation of histone H3.1 suppresses the lineage potential of skeletal muscle.

Author information

1
Department of Advanced Medical Initiatives, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
2
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan CREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0021, Japan.
3
Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan.
4
Department Bioengineering, Graduate School of Engineering, Osaka City University, Osaka 558-8585, Japan.
5
Department of Advanced Medical Initiatives, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan CREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0021, Japan yohkawa@epigenetics.med.kyushu-u.ac.jp.

Abstract

Lineage potential is triggered by lineage-specific transcription factors in association with changes in the chromatin structure. Histone H3.3 variant is thought to play an important role in the regulation of lineage-specific genes. To elucidate the function of H3.3 in myogenic differentiation, we forced the expression of GFP-H3.1 to alter the balance between H3.1 and H3.3 in mouse C2C12 cells that could be differentiated into myotubes. GFP-H3.1 replaced H3.3 in the regulatory regions of skeletal muscle (SKM) genes and induced a decrease of H3K4 trimethylation (H3K4me3) and increase of H3K27 trimethylation (H3K27me3). Similar results were obtained by H3.3 knockdown. In contrast, MyoD-dependent H3.3 incorporation into SKM genes in fibroblasts induced an increase of H3K4me3 and H3K27me3. In mouse embryos, a bivalent modification of H3K4me3 and H3K27me3 was formed on H3.3-incorporated SKM genes before embryonic skeletal muscle differentiation. These results suggest that lineage potential is established through a selective incorporation of specific H3 variants that governs the balance of histone modifications.

PMID:
25539924
PMCID:
PMC4333396
DOI:
10.1093/nar/gku1346
[Indexed for MEDLINE]
Free PMC Article

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