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J Infect Dis. 2015 Jun 1;211(11):1745-56. doi: 10.1093/infdis/jiu832. Epub 2014 Dec 23.

Medroxyprogesterone Acetate Regulates HIV-1 Uptake and Transcytosis but Not Replication in Primary Genital Epithelial Cells, Resulting in Enhanced T-Cell Infection.

Author information

1
Deptartment of Pathology and Molecular Medicine McMaster Immunology Research Centre, McMaster University, Hamilton.
2
Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec City, Canada.
3
Department of Medical Genetics, University of Toronto.

Abstract

Although clinical and experimental evidence indicates that female sex hormones and hormonal contraceptives regulate susceptibility to human immunodeficiency virus type 1 (HIV-1) infection, the underlying mechanism remains unknown. Genital epithelial cells (GECs) are the first cells to encounter HIV during sexual transmission and their interaction with HIV may determine the outcome of exposure. This is the first report that HIV uptake by GECs increased significantly in the presence of the hormonal contraceptive medroxyprogesterone acetate (MPA) and progesterone and that uptake occurred primarily via endocytosis. No productive infection was detected, but endocytosed virus was released into apical and basolateral compartments. Significantly higher viral transcytosis was observed in the presence of MPA. In GEC and T-cell cocultures, maximum viral replication in T cells was observed in the presence of MPA, which also broadly upregulated chemokine production by GECs. These results suggest that MPA may play a significant role in regulating susceptibility to HIV.

KEYWORDS:

HIV replication; HIV transmission; HIV-1; female sex hormones; genital epithelial cells; hormonal contraceptives; inflammation; medroxyprogesterone acetate; transcytosis

PMID:
25538276
DOI:
10.1093/infdis/jiu832
[Indexed for MEDLINE]

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