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Nan Fang Yi Ke Da Xue Xue Bao. 2014 Dec;34(12):1758-62.

[Effects of Biejiajian Pills on Wnt signal pathway signal molecules β-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells].

[Article in Chinese]

Author information

1
Department of Traditional Chinese Medicine, 458 Hospital of PLA, Guangzhou 510602, China.E-mail: hjzwb@aliyun.com.

Abstract

OBJECTIVE:

To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma.

METHODS:

HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR.

RESULTS:

Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities of β-catenin/TCF4 complex.

CONCLUSION:

Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.

PMID:
25537897
[Indexed for MEDLINE]

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