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J Extracell Vesicles. 2014 Dec 22;3:26913. doi: 10.3402/jev.v3.26913. eCollection 2014.

Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.

Author information

1
Krefting Research Centre, University of Gothenburg, Göteborg, Sweden.
2
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Australia.
3
Department of Neurosurgery, University of California at San Diego, San Diego, CA, USA.
4
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
5
Cedars-Sinai Medical Center, Los Angeles, CA, USA.
6
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, UK.
7
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
8
Bioinformatics Institute, Singapore, Singapore.
9
Department of Biochemistry, La Trobe University, Melbourne, Australia.
10
The Warren Alpert Medical School of Brown University, Providence, RI, USA.
11
Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
12
Department of Cellular and Molecular Biology, Hiroshima University Institute of Biomedical & Health Sciences, Hiroshima, Japan.
13
Department of Biochemistry and Cell Biology, Faculty of Veterinary medicine, Utrecht University, Utrecht, The Netherlands.
14
Department of Molecular and Comparative Pathobiology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
15
INSERM U932, Institut Curie, 26 rue d'Ulm, 75005, Paris, France; clotilde.thery@curie.fr.

Abstract

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

KEYWORDS:

ectosomes; exosomes; extracellular RNA; extracellular vesicles; microparticles; microvesicles

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