Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine

Int Immunopharmacol. 2015 Feb;24(2):361-368. doi: 10.1016/j.intimp.2014.12.022. Epub 2014 Dec 20.

Abstract

The aim of this study was to determine the anti-inflammatory effects of preventive administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin or with flax-seed oil in the gut of rats, which developed chronic inflammation following administration of the pro-carcinogen N,N-dimethylhydrazine (DMH). After 28weeks administration of probiotic/prebiotic-containing diet, rats were killed and their colons were examined by immunohistological criteria, whereas cytokines were determined in the jejunal mucosa. Application of DMH triggered the production of pro-inflammatory cytokines IL-2, IL-6, IL-17, and TNF-α, expression of pro-inflammatory mediators NF-κB, COX-2 and iNOS and caused depletion of goblet cells. Supplementing the diet with L. plantarum and its combination with the prebiotic abolished DMH-induced inflammatory process in the jejunal mucosa by inhibiting the production of pro-inflammatory cytokines and by stimulation of anti-inflammatory IL-10 cytokine synthesis, whereas concentration of TGF-β1 was not influenced significantly. Diet prevented a decrease in goblet cell numbers but numbers of mast cells were lowered only moderately. However, combined treatment of rats with L. plantarum and flax-seed oil had no significant effect on the parameters examined, except for decreased expression of NF-κB, in comparison with the negative control. Results indicate that the preventive administration of probiotic L. plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin to rats with DMH-induced chronic inflammation can reduce inflammatory process in the jejunal and colon mucosa, probably indirectly, and involves down-regulation of synthesis of pro-inflammatory cytokines and suppression of NF-κB activity in mucosal cells.

Keywords: Cancer prevention; Chronic inflammation; Flax-seed oil; Gut immunity; Inulin; Lactobacillus plantarum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Carcinogens
  • Colon / drug effects
  • Colon / metabolism
  • Colon / pathology
  • Cyclooxygenase 2 / metabolism
  • Cytokines / metabolism
  • Dimethylhydrazines
  • Female
  • Inflammation / chemically induced
  • Inflammation / drug therapy
  • Inflammation / metabolism
  • Inflammation / pathology
  • Intestinal Diseases / drug therapy
  • Intestinal Diseases / metabolism
  • Intestinal Diseases / pathology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / pathology
  • Inulin / pharmacology
  • Inulin / therapeutic use*
  • Jejunum / drug effects
  • Jejunum / metabolism
  • Lactobacillus plantarum*
  • Male
  • NF-kappa B / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Prebiotics*
  • Probiotics / pharmacology
  • Probiotics / therapeutic use*
  • Rats, Sprague-Dawley

Substances

  • Anti-Inflammatory Agents
  • Carcinogens
  • Cytokines
  • Dimethylhydrazines
  • NF-kappa B
  • Prebiotics
  • dimazine
  • Inulin
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat
  • Cyclooxygenase 2
  • Ptgs2 protein, rat