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Dev Cell. 2014 Dec 22;31(6):784-800. doi: 10.1016/j.devcel.2014.11.029.

Systematic study of Drosophila microRNA functions using a collection of targeted knockout mutations.

Author information

1
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore.
2
Procter and Gamble International Operations SA Singapore Branch, Quantitative Sciences, Statistics Asia, 70 Biopolis Street, Singapore 138547, Singapore.
3
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore. Electronic address: scohen@sund.ku.dk.

Abstract

MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

PMID:
25535920
DOI:
10.1016/j.devcel.2014.11.029
[Indexed for MEDLINE]
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