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Gene. 1989 Sep 1;81(1):165-8.

Respiratory-competent yeast mitochondrial DNAs generated by deleting intergenic regions.

Author information

1
Research School of Biological Sciences, Australian National University, Canberra.

Abstract

Two respiratory-competent yeast strains having mitochondrial (mt) DNA characterized by single non-overlapping deletions, encompassing intergenic sequences, have been crossed. Diploid daughter clones have been screened by electrophoresis of mtDNA fragments, and a respiratory-competent clone (ER8.75), having a recombinant small mtDNA with both parental deletions, has been detected. ER8.75 mtDNA lacks around 20% of wild-type intergenic sequences, encompassing three ori/rep sequences. This mutant could be helpful in analyzing the organelle genome and, particularly, the function of intergenic sequences.

PMID:
2553538
DOI:
10.1016/0378-1119(89)90347-8
[Indexed for MEDLINE]

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