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Bioanalysis. 2014;6(24):3283-93. doi: 10.4155/bio.14.241.

Novel sandwich immunoassays for the measurement of total and active FGF21.

Author information

1
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.

Abstract

BACKGROUND:

FGF21 is involved in glucose and lipid metabolism. Our objective was to develop two novel sandwich immunoassays that measure active and total levels of FGF21 in human plasma.

RESULTS:

Both immunoassays utilized affinity-optimized monoclonal antibodies generated specifically for either the mid-domain or the intact C-terminus of the respective FGF21 peptides. Total FGF21 levels measured from normal human plasma samples ranged from 42 to 462 pg/ml, while active FGF21 levels ranged from 11 to 399 pg/ml. The data also suggested no significant differences in the concentrations of active FGF21 when measured from pre- and post-prandial samples.

CONCLUSION:

We have successfully developed sensitive assays to measure active and total FGF21, which show the majority of total FGF21 in plasma is active FGF21.

PMID:
25534786
DOI:
10.4155/bio.14.241
[Indexed for MEDLINE]

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